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<ul> <li><img src="/Skins/515143/images/dot.png" /><span>產品型號：</span></li> <li><img src="/Skins/515143/images/dot.png" /><span>廠商性質：</span>生產廠家</li> <li><img src="/Skins/515143/images/dot.png" /><span>更新時間：</span>2022-09-07</li> <li><img src="/Skins/515143/images/dot.png" /><span>訪問量：</span>186</li> </ul> <div class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯系我們</a> </div> </div> <div class="clear"></div> </div>  <div class="info_title relative"> <b>詳細說明：</b> </div> <p><p style="width: 99%; height: 34px;"><strong>BL21(DE3)大腸桿菌</strong></p><div class="tabledivclass" style="width: 99%; height: 34px;"><table align="left" style="max-width: 748px;"><tbody style="-webkit-tap-highlight-color: rgba(0, 0, 0, 0); box-sizing: border-box;"><tr class="firstRow"><td>培養基</td><td>基礎培養基（LB）：酵母膏 5.0g，蛋白胨 10.0g，NaCl 10.0g，蒸餾水 1.0L，pH 7.0。121℃，15min滅菌。</td></tr></tbody></table></div><p><br/></p><p>傳代方法：</p><ol class=" list-paddingleft-2" style="list-style-type: decimal;"><li><p>準備無菌液體試管 1 支（含培養基 5~10mL），90mm 平板 2 塊； 2. 西林瓶管壁消毒后，無菌環境下，依次打開鋁蓋與膠塞，吸取約 0.5mL 液體培養基打入瓶中，輕輕吹打溶解菌片； 3. 菌片充分溶解后，將溶解液全部吸出，打回液體試管混勻； 4. 從試管液體中吸取混合液涂布平板，200μL/個； 5. 將試管和平板全部置于上述指ding條件培養，禁止密封纏繞平板，18-24h 后觀察培養液明顯渾濁，平板菌落或菌苔生長； 6. 如菌種未生長，應繼續培養至 72h，培養好后按照試驗需求具體使用。</p><p>生長條件：37℃；18-24h；好氧</p><p>存儲條件：-20℃</p><p>安全等級：1</p><p>應用領域：用于蛋白表達</p><p>共享方式：公益性共享<br/></p></li></ol><br /> <br /> <div id="proorder"> <link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產品：</h4> <div class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="BL21(DE3)大腸桿菌" 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